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冷水浸提中药天门冬，提取物经丙酮分级，得到ＡＧ－ａ，ＡＧ－ｂ和ＡＧ－ｃ三个级份。ＡＧ－ｃ用ＤＥＡＥ—纤维素柱纯化，得到水洗脱级份ＡＧ－ｗ和０．５ＮＮａＣｌ洗脱级份ＡＧ－ｃ。凝胶柱层析和醋酸纤维薄膜电泳，均表明ＡＧ－ｃ为均一性糖蛋白。红外光谱显示，所有的天门冬糖蛋白级份均含有乙酰基团。β—消去反应检测，ＡＧ－ｃ中糖和蛋白质间的连接键为Ｏ—型糖肽键
Cold water was used to extract Chinese asparagus. The extracts were graded by acetone to obtain three fractions of AG-a, AG-b and AG-c. AG-c was purified with a DEAE-cellulose column to obtain water eluted fraction AG-w and 0.5N NaCl eluting fraction AG-c. Gel column chromatography and cellulose acetate membrane electrophoresis showed that AG-c is a homogenous glycoprotein. Infrared spectra showed that all aspartame protein fractions contained acetyl groups. Beta-elimination reaction detection, the linkage between sugar and protein in AG-c is O-glycopeptide bond