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Objective To investigate the effect of Ureaplasma Urealyticum (UU) on the expression of IL-1 secretion in rat Sertoli cells Material & Methods Isolated rat Sertoli cells were infected by living UU , UU super natants, inactivated UU of different dosage respectively. IL- 1 secretion by rat Sertoli cells is determined by using group t-test and F-test.Results UU infection, both living UU and supatant without UU, could inhibit the IL-1 secretion in rats Sertoli cells (P＜ 0. 01).Conclusion The infection of Sertoli cells by UU inhibit the biological function of the Sertoli cells in rat.