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目的:探讨预运动训练对帕金森病模型小鼠运动能力和肝、脑胰岛素样生长因子表达的影响。方法:32只雄性小鼠(8~9周龄)随机分为安静+生理盐水组、运动+生理盐水组、安静+MPTP(1-甲基-4-苯基-1,2,3,6-四氢吡啶)组和运动+MPTP组,每组8只。运动组连续5周,每天1次中等强度(12米/分,20分钟)跑台训练。第6周时,各组分别注射生理盐水或中等剂量MPTP(30mg/kg×2次)。各组分别于MPTP注射前1天,MPTP注射后第2、5、8天共进行4次行为学测试评分。于末次行为学测试后测试小鼠肝、脑胰岛素样生长因子mRNA表达。结果:运动+生理盐水组和运动+MPTP组小鼠肝、脑胰岛素样生长因子mRNA表达分别显著高于其对应的安静+生理盐水组和安静+MPTP组(P<0.05);爬杆和游泳测试第2、5、8天和悬挂测试第2天,安静+MPTP组行为学评分显著低于安静+生理盐水组(P<0.05),运动+MPTP组小鼠行为学评分显著高于安静+MPTP组(P<0.05)。结论:预运动训练可能通过增加肝、脑胰岛素样生长因子表达,提高小鼠对抗MPTP脑损伤作用,减少帕金森病模型小鼠运动能力下降。
Objective: To investigate the effect of pre-exercise training on exercise capacity and expression of hepatic and insulin-like growth factor (IGF-I) in mice with Parkinson’s disease. Methods: Thirty-two male mice (8-9 weeks old) were randomly divided into three groups: sedentary + saline group, exercise + saline group, sedation + MPTP (1-methyl-4-phenyl-1,2,3,6 - tetrahydropyridine) group and exercise + MPTP group, 8 rats in each group. Exercise group for 5 weeks, 1 times a day moderate intensity (12 m / min, 20 minutes) treadmill training. At week 6, each group was injected with saline or a moderate dose of MPTP (30 mg / kg x 2 times). The rats in each group were subjected to behavioral test 4 times on the 1st day before MPTP injection and on the 2nd, 5th and 8th day after MPTP injection. Mouse liver and brain insulin-like growth factor mRNA expression was tested after the last behavioral test. Results: The mRNA expressions of hepatic and brain insulin-like growth factor in the mice in the exercise + saline group and the exercise + MPTP group were significantly higher than those in the quiet + saline group and the quiet + MPTP group (P <0.05) On the 2nd, 5th, and 8th days of the test and on the 2nd day of the suspension test, the scores of Bent + MPTP group were significantly lower than those of the quiet + saline group (P <0.05) MPTP group (P <0.05). Conclusion: Pre-exercise training may increase the expression of insulin-like growth factor in liver and brain, increase the anti-MPTP brain injury in mice and decrease the exercise capacity of mice with Parkinson’s disease.