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目的:用高效液相色谱法同时测定复方茶碱麻黄碱片中可可碱、盐酸麻黄碱、茶碱和咖啡因的含量。方法:采用Diamonsil C18色谱柱(250mm×4.6 mm,5μm);流动相:甲醇-0.05 mol·L-1磷酸二氢钾溶液-三乙胺(25:75:0.1);流速:0.8 ml·min-1;检测波长:210nm。结果:可可碱进样量在0.06～0.39μg(r=0.999 6)、盐酸麻黄碱进样量在0.03～0.16μg(r= 0.999 6)、茶碱进样量在0.06～0.38μg(r=0.999 6)、咖啡因进样量在0.04～0.23μg(r=0.999 6)范围内呈良好的线性关系。平均回收率分别为100.3%(RSD=0.3%);100.2%(RSD=0.6%);100.2%(RSD=0.6%);100.7%(RSD=0.5%)。结论:本方法简便、准确、重现性好,可用于同时测定复方茶碱麻黄碱片中4组分的含量。
OBJECTIVE: To determine the content of theobromine, ephedrine hydrochloride, theophylline and caffeine in the compound theophylline ephedrine tablets by high performance liquid chromatography. METHODS: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol-0.05 mol·L-1 potassium dihydrogen phosphate solution and triethylamine (25:75: 0.1). The flow rate was 0.8 ml · min -1; detection wavelength: 210 nm. Results: The theobromine injection volume ranged from 0.06 to 0.39 μg (r = 0.999 6), ephedrine hydrochloride injection ranged from 0.03 to 0.16 μg (r = 0.999 6), theophylline injection ranged from 0.06 to 0.38 μg (r = 0.999 6). There was a good linear relationship between the injection volume of caffeine and 0.04 ~ 0.23μg (r = 0.999 6). The average recovery rates were 100.3% (RSD = 0.3%), 100.2% (RSD = 0.6%), 100.2% (RSD = 0.6%) and 100.7% (RSD = 0.5%) respectively. Conclusion: The method is simple, accurate and reproducible. It can be used for the simultaneous determination of 4 components in compound theophylline ephedrine tablets.