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目的分析我院新生儿病房分离的大肠埃希菌产ESBLs基因型,为临床合理用药提供依据.方法 ESBLs表型筛选、确证试验采用K-B法,采用PCR方法对ESBLs基因型进行检测.结果90株产ESBLs大肠埃希菌进行PCR引物扩增,TEM型引物扩增阳性79株,占87.8%;SHV型引物扩增阳性76株,占84.4%;CTX-M型引物扩增阳性90株,占100%;OXA型引物扩增阳性7株,占7.8%.而72株同时产TEM型、SHV型及CTX-M型3种酶.结论在本院新生儿科分离的大肠埃希菌中流行ESBLs的基因型主要是CTX-M型,且同时存在产3种酶,应引起临床重视.“,”Objective To investigate the genotype of ESBLs producing Escherichia coli isolated from neonatal unit. Methods K-B method was preformed to screen the ESBLs phaenotype, and the TEM, SHV, CTX-M, OXA genes were detected by PCR. Results Among 90 ESBLs-producing strains, 79 isolates were TEM positive, 76 isolates were SHV positive, 90 isolates were CTX-M positive, 7 isolates were OXA positive. The gene of TEM, SHV, CTX-M(or OXA) were found co-exitent in 72 strains. Conclusions The main genotype of ESBLs producing Escherichia coli isolated from neonatal unit is blaCTX-M. Most of strains carry 3 ESBLs gene.