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为研究卵巢切除、卵巢周期和妊娠对前列环素合成酶(PGIS)的影响,选择西方杂交母羊,通过静脉注射戊巴比妥钠施行安乐死,分离子宫和网膜动脉。前期研究制备非妊娠黄体期羊(子宫,n=4;网膜,n=8)和妊娠119～130d母羊(子宫,n=6;网膜,n=8)的整个子宫和网膜动脉(3～5mm),从其中的4只收集另外的动脉片段(2～4mm)进行PGIS的免疫组化定位;后期研究从非妊娠卵巢切除(n=6)、卵泡期(n=6)和黄体期(n=6)羊及妊娠母羊(妊娠120～130d,n=8)制备子宫和网膜动脉的内皮分离
To investigate the effect of ovariectomy, ovarian cycle and pregnancy on prostacyclin synthase (PGIS), western hybrid ewes were selected and euthanized by intravenous injection of sodium pentobarbital to isolate uterine and omental arteries. Previous studies prepared the uterus and omental arteries of non-pregnant luteal phase sheep (uterus, n = 4; omentum, n = 8) and 119-130 d of gestation (uterus, n = 6; omentum, n = 8) (N = 6), follicular phase (n = 6) and non-pregnant ovariectomized group (n = 6) Luteal phase (n = 6) sheep and pregnant ewes (120 to 130 days of gestation, n = 8) were prepared for endothelial separation of uterine and omental arteries