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目的以D-半乳糖所致雌性衰老模型小鼠为实验对象,观察哈蟆油胶囊(OR)对其生殖器官的保护作用。方法采用雌性昆明小鼠48只,随机分为OR高、低剂量组和阳性药对照组、模型对照组,每组12只,D-半乳糖颈背部皮下注射6周。另设一组12只,同样部位每日注射生理盐水,作为正常对照组。第3周开始灌胃给药,模型对照组和正常对照组灌胃植物油。最后2周逐只进行阴道脱落细胞涂片,使用瑞氏-姬姆萨染液染色后观察其阴道角化细胞变化,计数动情期天数。给药结束后收集标本,采用放免法测定血清雌二醇水平,测定卵巢和子宫指数,观察卵巢和子宫病理变化,检测卵巢子宫中SOD以及MDA的水平。结果OR明显提高老年小鼠体内雌激素水平,延长小鼠动情期;增加其卵巢和子宫指数,改善卵巢和子宫的病理改变,降低卵巢子宫中MDA含量,提高其SOD活力。结论OR能够延缓雌性小鼠生殖衰老,增强生殖器官抗氧化作用,从而改善其萎缩和衰老程度。
OBJECTIVE: To investigate the protective effect of Hatake oil capsule (OR) on reproductive organs of female model mice induced by D-galactose. Methods Forty-eight female Kunming mice were randomly divided into high-dose and low-dose groups and positive drug control group and model control group. Twelve rats in each group were injected with D-galactose on the back of the neck for 6 weeks. Another group of 12 rats was placed in the same site and injected with saline daily as a normal control group. At the beginning of the third week, the rats were intragastrically administered. The model control group and the normal control group were given gastric oil. Only vaginal exfoliated cells were smeared in the last 2 weeks. After staining with Wright-Giemsa stain, the vaginal keratinocyte changes were observed and the number of estrus days was counted. At the end of administration, specimens were collected, serum estradiol levels were measured by radioimmunoassay, ovarian and uterine indexes were measured, and pathological changes in the ovaries and uterus were observed to detect SOD and MDA levels in the uterus of the ovary. Results OR significantly increased the level of estrogen in aged mice and prolonged the estrous period of mice; increased their ovarian and uterine index, improved the pathological changes of the ovary and uterus, decreased the content of MDA in the uterus of ovaries, and increased its SOD activity. Conclusion OR can delay the reproductive aging of female mice and increase the antioxidation of reproductive organs, thus improving the degree of atrophy and aging.