论文部分内容阅读
目的:检测食管鳞状细胞癌患者外周血中辅助性T细胞17(T helper 17,Th17)和调节性T细胞(regulatory T cell,Treg)的表达,探讨食管鳞状细胞癌中Th17/Treg比例的变化与食管鳞状细胞癌转移的关系。方法:收集山东大学齐鲁医院2009年9月至2010年5月食管鳞状细胞癌患者37例,其中男22例、女15例,平均年龄(61±7.85)岁;23例健康志愿者为对照。流式细胞术检测外周血Th17的表达情况,分析Th17占CD3+T细胞的比例,同样流式细胞术检测外周血Treg的表达及其占CD4+T细胞的比例。分析Th17/Treg比例变化与食管鳞状细胞癌病理特征的相关性。结果:食管鳞状细胞癌患者外周血中Th17占CD3+T细胞的比例显著升高[(2.11±0.66)%vs(0.84±0.19)%,P<0.01],Treg占CD4+T细胞的比例也显著升高[(6.21±1.48)%vs(4.43±0.78)%,P<0.01];食管鳞状细胞癌患者转移组与未转移组相比,Th17比例明显升高[(2.42±0.55)%vs(1.61±0.51)%,P<0.01],而Treg比例无明显差异。食管鳞状细胞癌患者外周血中Th17/Treg比例显著高于正常对照组[(0.35±0.13)%vs(0.19±0.04)%,P<0.01];食管鳞状细胞癌患者淋巴结转移组与未转移组相比,Th17/Treg比例显著升高[(0.39±0.13)%vs(0.29±0.10)%,P<0.05]。结论:食管鳞状细胞癌患者外周血中Th17/Treg比例显著升高,可能参与食管鳞状细胞癌的浸润和转移。
Objective: To detect the expression of T helper 17 (Th17) and regulatory T cell (Treg) in peripheral blood of patients with esophageal squamous cell carcinoma (ESCC), and to explore the relationship between Th17 / Treg ratio in esophageal squamous cell carcinoma The relationship between the changes and esophageal squamous cell carcinoma metastasis. Methods: Thirty-seven patients with esophageal squamous cell carcinoma were collected from Qilu Hospital of Shandong University from September 2009 to May 2010, including 22 males and 15 females, with an average age of (61 ± 7.85) years; 23 healthy volunteers . Flow cytometry was used to detect the expression of Th17 in peripheral blood. The proportion of Th17 in CD3 + T cells was analyzed. Flow cytometry was also used to detect the expression of Treg in peripheral blood and its proportion in CD4 + T cells. The correlation between Th17 / Treg ratio and pathological features of esophageal squamous cell carcinoma was analyzed. Results: The proportion of Th17 to CD3 + T cells in esophageal squamous cell carcinoma was significantly higher than that of CD3 + T cells (2.11 ± 0.66% vs 0.84 ± 0.19%, P <0.01) (6.21 ± 1.48)% vs (4.43 ± 0.78)%, P <0.01]. The proportion of Th17 in esophageal squamous cell carcinoma was significantly higher than that in the non-metastatic group (2.42 ± 0.55) % vs (1.61 ± 0.51)%, P <0.01], while the proportion of Treg had no significant difference. The proportion of Th17 / Treg in peripheral blood of patients with esophageal squamous cell carcinoma was significantly higher than that of the normal control group [(0.35 ± 0.13)% vs (0.19 ± 0.04)%, P <0.01] The proportion of Th17 / Treg was significantly higher in the metastatic group than in the metastatic group [(0.39 ± 0.13)% vs (0.29 ± 0.10)%, P <0.05]. Conclusion: The proportion of Th17 / Treg in peripheral blood of patients with esophageal squamous cell carcinoma is significantly increased, which may be involved in the invasion and metastasis of esophageal squamous cell carcinoma.