论文部分内容阅读
目的探讨Toll样受体4(TLR4)在军团菌感染中作用。方法用C3H/HeJ小鼠、C3H/HeN小鼠作为实验动物,实验设C3H/HeJ、C3H/HeN染菌组及C3H/HeN对照组,染菌组气管注射军团菌悬液建立军团菌感染模型,对照组气管注射无菌生理盐水;观察主要脏器系数及肺组织病理改变,流式细胞术测定各组小鼠外周血单个核细胞(PBMC)中TLR4蛋白表达量变化。结果肺组织病理切片显示染菌组均有炎性细胞浸润并出现肺泡血管出血现象;与C3H/HeN对照组比较,染菌72 h C3H/HeJ、C3H/HeN染菌组小鼠肺脏、脾脏脏器系数均升高,差异有统计学意义(P<0.05);染菌12 h时,C3H/HeJ染菌组小鼠PBMC中TLR4蛋白表达量为(48.31±5.22),低于C3H/HeN染菌组的(113.70±17.74)(P<0.05),C3H/HeN染菌组与C3H/HeN对照组TLR4蛋白表达量差异无统计学意义(P>0.05)。结论 TLR4基因突变使小鼠对军团菌反应性降低,但TLR4对军团菌的抗感染作用机制仍需进一步研究。
Objective To investigate the role of Toll-like receptor 4 (TLR4) in Legionella infection. Methods C3H / HeJ mice and C3H / HeN mice were used as experimental animals. Legionella pneumophila suspension was injected into C3H / HeJ, C3H / HeN and C3H / HeN control groups. . The control group was injected with sterile saline into the trachea. The major organ coefficients and pathological changes of lung tissue were observed. The expression of TLR4 protein in peripheral blood mononuclear cells (PBMC) of each group was measured by flow cytometry. Results Compared with C3H / HeN control group, inflammatory cell infiltration and alveolar vascular hemorrhage were found in the lung tissue. The lungs and spleen of mice infected with C3H / HeJ and C3H / HeN cells at 72 h (P <0.05). At 12 h after infection, the expression of TLR4 protein in PBMC of C3H / HeJ-infected mice was (48.31 ± 5.22), lower than that of C3H / HeN stain (113.70 ± 17.74) (P <0.05). There was no significant difference in TLR4 protein expression between C3H / HeN and C3H / HeN control groups (P> 0.05). Conclusion The mutation of TLR4 gene reduces the reactivity of mice to Legionella, but the mechanism of anti-infective effect of TLR4 against Legionella still needs further study.