【摘 要】
：
In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and t
【机 构】
：
Animal Science and Technology College
论文部分内容阅读
In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and the development of preimplantation embryos were studied. The possible effective targets of icariin promoting preimplantation embryo development in vitro and anti-apoptosis were determined. The embryos were cultured in modified CZB medium (mCZB) as control group. The experimental group (Ica group) was supplemented with 0.6μg mL-1 icariin. Mouse pronuclear embryos were cultured in vitro until blastocysts. The development rates of preimplantation embryos were observed. The total cell number, apoptotic cell number and the rate of apoptotic cells in blastocysts were analysed by the staining of Hoechst33342 and labeling of TUNEL and detected under a laser confocal scanning microscope. The miRNA-21 expression, the mRNA levels of pro-apoptotic Caspase3, and the target genes of miRNA-21:pro-apoptotic PTEN, anti-apoptotic Bcl-2 were detected by real-time RT-PCR. The results showed that percentages of morulaes and blastocysts in Ica group were both extremely higher than control group ((85.14±6.57)%vs. (72.04±11.58)%;(82.50±7.11)%vs. (66.80±11.70)%, respectively, P
其他文献
In the case of a previous offspring with trisomy 21,recurrence risk for Down syndrome is about 1%.1 It may be due to chance, but the possibility of germline mos
The cellular immunity of current influenza split vaccine is relatively low. It is necessary to develop a novel vaccine to improve the cellular immunity. The aut
Inflammatory myofibroblastic tumor (IMT) of the alimentary tract often occurs in children or young adults,but may occur at any age. Symptoms are nonspecific and
AIM: To study the relationship between inflammatory response and liver regeneration (LR) at transcriptional level.METHODS: After partial hepatectomy (PH) of rat
Increasing K+adsorption can be an effective alternative in building an available K pool in soils to optimize crop recovery and minimize losses into the environm
The discovery of cell-free fetal DNA in maternal plasma in 1997 has opened up new possibilities for noninvasive diagnosis.1 By RT-PCR, circulating fetal DNA can
Pupillary constriction occurs with intravenous administration of opioids such as sufentanil.1 However, few studies have investigated the pupillary effects of in
A new method for the enrichment of Strychnos alkaloids in biological samples via liquid-phase rnicroextraction (LPME) based on porous polypropylene hollow fiber
The panel of serologic markers for inflammatory bowel diseases (IBD) is rapidly expanding. Although anti-Saccharomyces cerevisiae antibodies (ASCA) and atypical