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目的对牡荆Viter negundo var.cannabifolia子及黄荆Vitex negundo子进行比较研究,为其鉴别提供实验依据。方法采用原植物外观、性状、显微、薄层和HPLC鉴别方法,对黄荆子与牡荆子进行定性定量研究。结果黄荆子与牡荆子在原植物形态、药材性状上相似,通过原植物和性状进行鉴别存在一定困难;显微鉴别中石细胞特征可为两者的有效鉴别提供实验依据;薄层鉴别中以黄荆子素为对照品,黄荆子中黄荆子素丰度明显高于牡荆子;HPLC测定中,黄荆子中所含黄荆子素的量高于牡荆子。结论可采用显微、薄层和HPLC方法,结合药材粉末的石细胞特征和黄荆子素量差异对黄荆子与牡荆子进行有效地鉴别。
Objective To compare the Vitex negundo seed of Vite negundo var.cannabifolia and Vitex negundo, and provide experimental basis for its identification. Methods Qualitative and quantitative analysis of P. xanthophyllotorum and P. vietnameseum was carried out using the original plant appearance, characters, microscopic, TLC and HPLC methods. Results The results showed that there were some difficulties in the identification of original plant morphology and medicinal materials between the original plant morphology and the medicinal materials. The identification of stone cells in the microscopic identification could provide an experimental basis for the effective identification of the two. As reference substance, the vitexin content of vitexin was obviously higher than that of vitexin. The content of vitexin in vitexin was higher than that of vitentin in HPLC. Conclusion The microscopic, TLC and HPLC methods can be used to effectively identify P. xanthophyllotorum and P. vietnamicum in combination with the characteristics of the stone cells and the content of P. viridis.