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目的建立模拟人的原位裸小鼠模型,评价抗人膀胱癌免疫毒素临床前的疗效。方法将体外培养的人移行细胞癌细胞系(BIU-87)原位移植于经酸处理的裸小鼠膀胱内,定期用核磁共振成像(MRI)检查,并进行解剖、组织学检查和免疫细胞化学的检测。结果在第7～36天内,成瘤率为92．9％(52／56);其中第12～13天处死的裸小鼠中,95．2％(40／42)发展成移行细胞癌,以浅表性为主。组织学检查证实为Ⅱ～Ⅲ级;免疫细胞化学证实,该模型具有BIU-87细胞的特征。在肿瘤的发生发展中,MRI检查的结果与组织学检查的结果密切相关。接种后第7～9天可以检测出原位癌(CIS),其后发展成乳头状瘤或浸润性疾病。结论模拟人的原位裸小鼠模型重复性好,是临床前研究膀胱灌注治疗的理想模型。
Objective To establish a mimic human orthotopic nude mouse model to evaluate the preclinical efficacy of anti-human bladder cancer immunotoxin. Methods Human transitional cell carcinoma cell line (BIU-87) cultured in vitro was transplanted in situ in the bladder of acid-treated nude mice. The cells were examined regularly by magnetic resonance imaging (MRI) and dissected, histologically examined and immunocytes Chemical testing. Results The tumorigenic rate was 92.9% (52/56) in the 7th to 36th days. 95.2% (40/42) of the nude mice sacrificed on the 12th to 13th days developed into transitional cell carcinoma, Mainly superficial. Histological examination confirmed grade Ⅱ ~ Ⅲ; immunocytochemistry confirmed that the model has the characteristics of BIU-87 cells. In the development of tumors, the results of MRI examination are closely related to the results of histological examination. In situ carcinomas (CIS) can be detected from day 7 to day 9 after inoculation and subsequently develop into papilloma or infiltrative disease. Conclusions Simulated human orthotopic nude mouse model has good repeatability and is an ideal model for the preclinical study of bladder irrigation.