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为转移野生种的有利性状,进行了小麦体细胞不对称杂交的研究。由小麦品种“京花1号”和多花黑麦草的悬浮系分离原生质体通过电融合得到了大量的再生愈伤组织,并分化出了99株绿苗和大量白化苗。同工酶检测表明,所得到的植株带有多花黑麦草的部分遗传性状,是真杂种。为抑制亲本原生质体的分裂,分离原生质体前,多花黑麦草的悬浮细胞用软X-射线照射,以杀死部分细胞核;小麦的原生质体用2～4mmol/L的IOA室温暗处理15min。研究表明,受体悬浮系的再生能力愈强,融合的愈伤组织的再生能力也愈强,白化苗的再生频率也相对较低。
In order to transfer the favorable traits of wild species, the asymmetric hybridization of wheat somatic cells was studied. A large amount of regenerated callus was obtained by electrosynthesis separation of protoplasts from wheat cultivars “Jinghua 1 ” and suspension plants of pollen ryegrass, and 99 green shoots and a large number of albino seedlings were differentiated. Isoenzyme analysis showed that the resulting plants with parthenogenetic ryegrass genetic characteristics are true hybrids. In order to restrain the protoplast from dividing, protoplasts of ryegrass were irradiated with soft X-rays to kill some nuclei before the protoplasts were isolated. The protoplasts of wheat were dark-treated with 2 ~ 4mmol / L IOA at room temperature for 15min. Studies have shown that the stronger the regenerative capacity of the recipient suspension system, the stronger the regenerative capacity of the fused callus, and the regeneration frequency of albino seedlings is relatively low.