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目的:观察复方楂金颗粒(CZJG)中试产品对高脂饲料诱导的非酒精性脂肪肝炎大鼠的影响,探讨其防治机制。方法:SD大鼠60只,随机分为正常组、模型组、易善复组(0.06 g/kg)与复方楂金高、中、低剂量组(3.50、1.75、0.88g/kg),每组10只。除正常组以标准饲料喂养外,其余各组均以高脂饲料喂养造模。12周末称重,处死所有大鼠,计算肝指数。测定大鼠血清中ALT、AST、ALP、GGP、TC、TG、HDL-C、LDL-C的水平;ELISA法检测大鼠血清中MDA、SOD、GSH-PX、TAOC、TNF-α、Leptin的活性;并对大鼠肝组织作病理学检查。结果:高、中剂量组CZJG大鼠肝脏炎症程度明显减轻(P<0.01);与模型组比较,CZJG高、中剂量组血清中TG、ALT、AST、ALP水平显著降低(P<0.05;P<0.01);高、中、低剂量组血清中TC、LDL-C、TNF-α、Leptin的水平显著降低(P<0.05;P<0.01);中、低剂量组血清中SOD、GSH-PX酶的活性显著升高(P<0.05;P<0.01);高、低剂量组显著降低MDA活性显著下降(P<0.01)。结论:CZJG中试颗粒可有效防治实验性非酒精性脂肪肝,其机制可能为:减少TNF-α、Leptin的产生;提高抗脂质过氧化能力。
Objective: To observe the effects of CZJG pilot product on non-alcoholic steatohepatitis induced by high-fat diet and its mechanism of prevention and treatment. Methods: Sixty Sprague Dawley rats were randomly divided into three groups: normal group, model group, Yishen Fu group (0.06 g / kg), and high, medium and low doses of compound hawthorn (3.50,1.75,0.88g / kg) Group of 10. Except normal group fed with standard feed, all the other groups were fed with high fat diet. Weigh 12 weeks, all rats were sacrificed, calculate the liver index. The levels of ALT, AST, ALP, GGP, TC, TG, HDL-C and LDL-C in the serum of the rats were measured. The levels of MDA, SOD, GSH-PX, TAOC, TNF- Activity; and rat liver tissue for pathological examination. Results: Compared with model group, serum levels of TG, ALT, AST and ALP in CZJG high and medium dose groups were significantly decreased (P <0.05; P <0.01). Serum levels of TC, LDL-C, TNF-αand Leptin in high, middle and low dose groups were significantly decreased (P <0.05; P <0.01) (P <0.05; P <0.01). The activity of MDA decreased significantly in high and low dose groups (P <0.01). CONCLUSION: CZJG Pilot Test can effectively prevent and treat experimental non-alcoholic fatty liver disease. Its mechanisms may include: reducing the production of TNF-α and Leptin; and improving the anti-lipid peroxidation ability.