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单克隆抗体M26-32是一株泛种特异性的单抗，但其有效成份尚不清楚。为此我们采用饱和硫酸铁沉淀法、葡聚精凝胶（Sephadex）G200过柱法和MonoQ阴离子交换树脂层析法对合M)26-32)单抗的小鼠腹水进行纯化，并比较其纯化的效果。材料与方法1材料1．1M26－32细胞株中国科学院基础研
Monoclonal antibody M26-32 is a pan-specific monoclonal antibody, but its active ingredient is not yet known. To this end, we purified the ascitic fluid of mouse M (26-32) monoclonal antibody by saturated ferric sulfate precipitation method, Sephadex G200 column chromatography and MonoQ anion exchange resin chromatography, and compared them Purification effect. Materials and Methods 1 Materials 1.1M26-32 cell line Basic Research Institute of Chinese Academy of Sciences