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目的观察噪声对小鼠学习记忆及脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法将144只昆明小鼠,随机分为对照组、80 dB(A)和100 dB(A)噪声组,噪声持续暴露1个月。噪声停止后第1、20、30和40天,分别用Morris水迷宫测试小鼠学习记忆,黄嘌呤氧化酶法测定SOD活力,硫代巴比妥酸比色法测定MDA含量。结果噪声停止时,3组小鼠逃避潜伏分别为(38.35±12.44)、(68.49±17.88)和(89.32±22.62)s,跨台次数分别为(7.25±1.91)、(4.32±1.28)和(3.01±1.01)次,SOD活力分别为(281.53±110.66)、(166.98±61.42)和(104.18±39.12)kU/g,MDA含量分别为(1.23±0.80)、(2.68±1.10)、(4.16±1.32)μmol/g,各指标组间比较差异均有显著性(P<0.05)。噪声停止后第30天,80 dB(A)噪声组小鼠上述指标与对照组比较,差异无显著性(P>0.05);噪声停止后第40天,3个组间的各指标比较,差异均无显著性(P>0.05)。结论噪声能降低小鼠脑组织SOD活力,增加MDA含量,降低小鼠学习记忆。脱离噪声后,小鼠脑组织SOD活力和学习记忆均能恢复,噪声强度越大,恢复需要的时间越长。
Objective To observe the effect of noise on learning and memory and the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in brain of mice. Methods 144 Kunming mice were randomly divided into control group, 80 dB (A) and 100 dB (A) noise group, and the noise was continuously exposed for 1 month. On day 1, day 20, day 30, and day 40 after stop of the noise, the learning and memory of mice were measured by Morris water maze, the activity of SOD was measured by xanthine oxidase method, and the content of MDA was measured by thiobarbituric acid colorimetry. Results When the noise was stopped, the escape latency of the three groups of mice was (38.35 ± 12.44), (68.49 ± 17.88) and (89.32 ± 22.62) s, respectively, and the number of cross-platform trips was 7.25 ± 1.91 and 4.32 ± 1.28 respectively 3.01 ± 1.01), SOD activities were (281.53 ± 110.66), (166.98 ± 61.42) and (104.18 ± 39.12) kU / g, MDA contents were 1.23 ± 0.80, 2.68 ± 1.10 and 4.16 ± 1.32) μmol / g, there was significant difference between each index group (P <0.05). On the 30th day after the noise was stopped, there was no significant difference in the above indexes between the 80 dB (A) noise group and the control group (P> 0.05); on the 40th day after the noise stopped, the differences among the three groups No significant (P> 0.05). Conclusion Noise can reduce the activity of SOD in brain tissue, increase the content of MDA and decrease the learning and memory of mice. After being out of the noise, the SOD activity and learning and memory of the mouse brain tissue can be recovered. The greater the noise intensity, the longer the recovery time will be.