一、细胞小片的制备 (一)细胞小片制备原则 1、取片部位制备细胞小片的取片部位限定为外套痕以外的边缘膜。即从前闭壳肌起一直到后闭壳肌背部的边缘壳翼膜。 2、细胞小片的规格通常分为大中小三种。大片为5～6×5～6mm~2,中片为4～5×4～5mm~2,小片为2～3×2～3mm~2。 3、制片蚌的年龄以1龄蚌最佳,2龄蚌次之。3龄以上性成熟蚌不宜用于 First, the preparation of small pieces of cells (a) the principle of the preparation of small pieces of cells 1, taking the chip parts of the preparation of small pieces of the film is limited to the outer margin of the margin film. That is, the frontal shell muscle up to the back of the posterior capsular muscle edge of the shell membrane. 2, the specifications of small pieces of cells are usually divided into three kinds of medium and small. Large for the 5 ~ 6 × 5 ~ 6mm ~ 2, the film is 4 ~ 5 × 4 ~ 5mm ~ 2, small pieces of 2 ~ 3 × 2 ~ 3mm ~ 2. 3, mussel production age to 1 mussel best, followed by 2 mussels. Age 3 or more mature mussels should not be used