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目的研究溶血磷脂酸(lysophosphatidic acid,LPA)对肝癌细胞MHCC97H迁移行为的影响,并探究其相关的分子机制。方法采用Transwell法检测LPA处理后MHCC97H细胞迁移的变化情况,并通过Rho相关蛋白激酶(ROCK)抑制剂Y-27632检测ROCK信号通路在其中的作用,利用免疫荧光染色和免疫印迹法检测LPA对MHCC97细胞骨架F-actin表达的影响,通过原子力显微镜检测LPA作用后MHCC97H细胞弹性模量的变化。结果LPA显著促进MHCC97H的迁移,ROCK抑制剂Y-27632可阻断LPA诱导的MHCC97H细胞迁移。LPA上调MHCC97H中F-actin的表达,减小MHCC97细胞的弹性模量。结论 LPA可能主要通过ROCK/F-actin通路降低肝癌细胞MHCC97H的硬度,从而促进其迁移行为。
Objective To investigate the effect of lysophosphatidic acid (LPA) on the migration of hepatocellular carcinoma cell line MHCC97H and to explore its related molecular mechanism. Methods The migration of MHCC97H cells treated with LPA was detected by Transwell method. The effect of ROCK signaling pathway was detected by Rho inhibitor Y-27632. Immunofluorescence staining and immunoblotting were used to detect the expression of MHCC97H Cytoskeletal F-actin expression was examined by atomic force microscopy after the change of the elastic modulus of MHCC97H cells after LPA. Results LPA significantly promoted the migration of MHCC97H. ROCK inhibitor Y-27632 could block LPA-induced MHCC97H cell migration. LPA up-regulated the expression of F-actin in MHCC97H and decreased the elastic modulus of MHCC97 cells. Conclusions LPA may decrease the hardness of MHCC97H cells through ROCK / F-actin pathway and promote its migration.