目的 :研究太白楤木根皮中齐墩果酸的含量测定方法。 方法 :采用紫外分光光度法、薄层扫描法及高效液相色谱法(HPL C)测定 ,并就测定数据进行比较。 结果 :紫外分光光度法操作简便 ,稳定性好 ,但不精确 ;薄层扫描法影响因素多 ,数据稳定性差 ;HPL C法针对单一成分测定 ,数据稳定 ,可信度高。 结论 :不同方法所得结果差异很大 ,RP- HPL C法为太白楤木中齐墩果酸含量测定的最佳方法 Objective : To study the determination method of oleanolic acid in root bark of Eucalyptus urophylla. Methods : Ultraviolet spectrophotometry, thin-layer scanning and high-performance liquid chromatography (HPL C) were used for the determination and the data were compared. RESULTS: UV spectrophotometry was easy to operate, with good stability but inaccurate; thin layer scanning method had many influence factors and poor data stability; HPL C method was used for single component determination, and the data was stable and reliable. Conclusion : The results obtained by different methods are quite different. RP-HPL C method is the best method for determination of oleanolic acid in Eucalyptus urophylla