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目的:探讨不同钙化合物对大鼠铝、铅代谢的影响。方法:将幼年Sprague-Dawley大鼠30只(雌雄性各半),分成柠檬酸钙+铝+铅组、醋酸钙+铝+铅组和碳酸钙+铝+铅组等3组。各组动物分别用不同形式的钙化合物通过灌胃给予等剂量钙元素,共4周。实验结束后,用电感耦合等离子体原子发射光谱法(ICP-AES)分别测定全血、肝、肾、股骨和大脑中铝和铅的含量。结果:醋酸钙组、柠檬酸钙组和碳酸钙组铅的表观吸收率分别为(-29.97±28.85)%,(-86.20±87.60)%和(-163.56±93.77)%;大脑中铝浓度分别为(3.84±1.49),(3.67±1.31)和(0.83±1.61)μg/g。大鼠摄入醋酸钙和柠檬酸钙后对铅的表观吸收率要高于碳酸钙组的大鼠(F=-23.744,7.802,P<0.05)。摄入柠檬酸钙和醋酸钙的大鼠的大脑和骨骼组织中铝和铅水平高于摄入碳酸钙的大鼠(F=12.411～38.333,P<0.05)。结论:长期摄入柠檬酸钙和醋酸钙有增加铅吸收的危险,并可使铅和铝蓄积在骨骼和大脑。
Objective: To investigate the effects of different calcium compounds on aluminum and lead metabolism in rats. Methods: 30 young Sprague-Dawley rats were divided into three groups: calcium citrate + aluminum + lead group, calcium acetate + aluminum + lead group and calcium carbonate + aluminum + lead group. Each group of animals were given different doses of calcium compounds by different forms of calcium compounds by gavage for 4 weeks. After the experiment, the contents of aluminum and lead in whole blood, liver, kidney, femur and brain were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). Results: The apparent absorption rates of Pb in calcium acetate group, calcium citrate group and calcium carbonate group were (-29.97 ± 28.85)%, (-86.20 ± 87.60)% and (-163.56 ± 93.77)%, respectively. (3.84 ± 1.49), (3.67 ± 1.31) and (0.83 ± 1.61) μg / g, respectively. The apparent absorption rate of Pb after calcium acetate and calcium citrate intake in rats was higher than that in calcium carbonate group (F = -23.744,7.802, P <0.05). The levels of aluminum and lead in brain and skeletal tissues of rats consuming calcium citrate and calcium acetate were higher than those of rats consuming calcium carbonate (F = 12.411-38.333, P <0.05). Conclusion: Long-term intake of calcium citrate and calcium acetate increases the risk of lead absorption and allows lead and aluminum to accumulate in the bones and brain.