论文部分内容阅读
鉴于大量获得人血清较困难,价又昂贵,取自不同供血者的血清培养效果不一,在疟区所得血清含疟疾抗体者较多,乙型病毒性肝炎的发病率较高致有潜在的危险性等原因,作者进行了寻找价廉而又效果满意的人血清的代用品的试验。材料及方法:恶性疟原虫FCR-3株,以Trager及Jensen氏蜡烛缸培养皿法培养。60×15nun皿中含5ml培养物。用A型CPD血,培养时压积细胞为10%或12%。对照组均以RPMI 1640、Hepes及10%人血清培
In view of the difficulties of obtaining human serum in large quantities, the price is expensive, serum culture effects from different blood donors are different, and the serum malaria antibodies obtained from malaria patients are more, and the incidence of hepatitis B virus is higher, resulting in potential Dangerous and other reasons, the author conducted a search for inexpensive and satisfactory human serum substitutes. MATERIALS AND METHODS: Plasmodium falciparum FCR-3 strain was cultured by Trager and Jensen candle jar culture. A 5 x culture was made in 60 x 15nun dishes. With type A CPD blood, 10% or 12% of the cells were compressed during culture. Control groups were RPMI 1640, Hepes and 10% human serum culture