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短暂禁食可引起包括糖皮质激素释放在内的一系列神经内分泌改变,对于疾病防治具有独特而重要的意义。本研究观察了短暂禁食对四氯化碳(carbon tetrachloride,CCl_4)诱导的急性肝炎小鼠肝组织损伤程度及炎症反应水平的影响及其与糖皮质激素之间的可能关联。结果发现,短暂禁食可明显下调CCl_4处理小鼠血浆中天冬氨酸转氨酶(aspartate transaminase,AST)及丙氨酸转氨酶(alanine transaminase,ALT)水平,显著减轻肝组织病理学改变,对肝组织内还原型谷胱甘肽(glutathione,GSH)含量无明显影响但可显著降低血浆中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白介素-6(interleukin-6,IL-6)浓度。糖皮质激素受体拮抗剂米非司酮不能阻断短暂禁食对转氨酶水平的下调作用,糖皮质激素受体激活剂地塞米松也不能模拟短暂禁食对转氨酶水平的下调作用。以上结果提示:短暂禁食可通过不依赖于糖皮质激素的途径抑制炎症反应、从而减轻CCl_4诱导的急性肝炎。
Short-term fasting can cause a series of neuroendocrine changes including glucocorticoid release, which has unique and important significance for the prevention and treatment of diseases. This study examined the effects of brief fasting on liver tissue injury and inflammatory response in acute hepatitis C induced by carbon tetrachloride (CCl 4) and its possible relationship with glucocorticoids. The results showed that short-term fasting could significantly reduce the levels of aspartate transaminase (AST) and alanine transaminase (ALT) in the plasma of CCl_4-treated mice, significantly reduce the pathological changes of liver tissue, There was no significant effect on the contents of glutathione (GSH) but significantly decreased the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentration. Glucocorticoid receptor antagonist mifepristone can not block the short-term fasting on the role of aminotransferase levels, glucocorticoid receptor activator dexamethasone can not simulate the transient fasting on the role of transaminase down. The above results suggest that short-term fasting can reduce the CCl 4 -induced acute hepatitis by inhibiting the inflammatory response through the glucocorticoid-independent pathway.