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马铃薯抗菌肽SN1是一种新型抗菌肽。为明确SN1是否抑制小麦重要土传真菌小麦纹枯菌(禾谷丝核菌)、小麦根腐菌(平脐蠕孢菌)的生长,本文克隆了马铃薯抗菌肽基因SN1的全长编码序列,将该基因编码序列亚克隆到原核表达载体pGEX-4T-1上,构建成GST-SN1融合蛋白表达载体。经异丙基-β-D-硫代半乳糖苷(IPTG)诱导,获得了以包涵体形式表达的GST-SN1重组蛋白。经过裂解、洗涤、溶解、复性等处理,获得了纯化的GST-SN1融合蛋白。体外抑菌试验表明,SN1显著抑制禾谷丝核菌、平脐蠕孢菌菌丝生长,可作为上述植物病害抗性育种的潜在基因。
Potato antimicrobial peptide SN1 is a new antimicrobial peptide. In order to determine whether SN1 could inhibit the growth of wheat root rot fungi (Rhizoctonia cerealis and Rhizoctonia cerealis) which are important soil-borne fungi of wheat, we cloned the full-length coding sequence of potato antitumor peptide SN1, The coding sequence of the gene was subcloned into prokaryotic expression vector pGEX-4T-1 to construct GST-SN1 fusion protein expression vector. After induced by isopropyl-β-D-thiogalactopyranoside (IPTG), GST-SN1 recombinant protein was obtained as inclusion body. After cleavage, washing, dissolving, refolding and other treatments, the purified GST-SN1 fusion protein was obtained. In vitro bacteriostasis test showed that SN1 significantly inhibited the growth of R. solani and N. urophylla mycelia and could be used as potential genes for plant disease resistance breeding.