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应用随机扩增多态性DNA (RAPD)技术对茶树无性系品种“晚绿”进行亲子关系分析。所用 10 8条随机引物中的 93条引物对日本静冈和枕畸两地取样的 6个无性系茶树品种的 8份材料的基因组DNA分别扩增出 1- 8条谱带 ,平均每条引物 3 5条。其中 19条引物扩增出“晚绿”与其母本“数北”不同的RAPD分子标记 30个。从中选择 6条引物对 8份供试材料的基因组DNA鉴定表明 ,其中 4个RAPD分子标记显示出“晚绿”品种特异性 ,说明包括“静在 16”在内的 4个供试品种都不是“晚绿”的真正父本。本文还证明 ,不同生态条件下生长的同一无性系茶树品种的基因组DNA具有遗传保守性
Parental relationship analysis of “late green”, a clonal variety of tea tree, was conducted using random amplified polymorphic DNA (RAPD) technique. Ninety primers from 10 8 random primers were used to amplify 8 to 8 bands from 8 genomic DNA samples of 6 clones of tea varieties sampled from Shizuoka and Pillow respectively. The average of each primer 3 5 articles. Among them, 19 primers amplified 30 RAPD markers different from “Late Green” and “North” of their female parent. Six primers were selected to identify the genomic DNA of the eight tested materials. Four of the RAPD markers showed “late green” variety specificity, indicating that all the four tested varieties including “static at 16” were not The real father of “late green”. It is also demonstrated that genomic DNA of the same clonal tea cultivars grown under different ecological conditions is genetically conserved