论文部分内容阅读
目的建立了饮用水中甲硝唑的固相萃取-超高效液相色谱串联质谱(UPLC-MS-MS)测定法。方法将200 ml水样经HLB固相萃取柱进行净化浓缩,利用T3色谱柱分离,以甲醇和0.2%甲酸水溶液为流动相,采用超高效液相色谱串联质谱(UPLC-MS-MS)法测定甲硝唑的浓度。结果在0.006~500 ng/ml的线性范围内,所得甲硝唑的回归方程为y=6 340x+23 600,线性关系良好,r=0.999 6。方法的检出限为1.9 ng/L,定量下限为6.0 ng/L,回收率为91.3%~99.2%,RSD<6.0%。结论该方法灵敏度高,选择性强,适用于饮用水中甲硝唑的测定。
Objective To establish a solid-phase extraction-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method for the determination of metronidazole in drinking water. Methods 200 ml water samples were purified by HLB SPE column and separated by T3 column. The mobile phase consisted of methanol and 0.2% formic acid as mobile phase, and determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) Metronidazole concentration. Results The linear regression equation of metronidazole was y = 6 340x + 23 600 in the linear range of 0.006 ~ 500 ng / ml with good linearity (r = 0.999 6). The detection limit of the method was 1.9 ng / L, the lower limit of quantification was 6.0 ng / L, the recovery was 91.3% -99.2%, and the RSD was less than 6.0%. Conclusion The method is sensitive and selective and suitable for the determination of metronidazole in drinking water.