论文部分内容阅读
【目的】研究老化处理对大麦种子活力及其萌发早期胚乳淀粉粒类型、淀粉含量、淀粉酶活性及内源激素的影响。【方法】采用高温高湿的人工老化处理方法。【结果】人工老化使4个大麦品种的发芽特性指标和种胚活力均逐渐降低。萌发72 h的种子胚乳中小淀粉粒数目增加,大淀粉粒数目减少,淀粉质量分数增加。随着老化时间的延长,α-淀粉酶、β-淀粉酶和极限糊精酶活性均呈降低的趋势,且老化15 d的种子淀粉酶活性显著低于对照;萌发24 h,GA_3含量显著低于对照,萌发48~72 h,GA_3含量均高于对照。ABA含量在萌发过程中均高于对照。【结论】人工老化打破了萌发过程中GA_3和ABA间的平衡,导致淀粉酶活性降低,淀粉分解减缓,进而使大麦种子活力降低,萌发受到抑制。
【Objective】 The objective of this study was to investigate the effect of aging treatment on the seed vigor of barley and the types of starch granules, starch content, amylase activity and endogenous hormones in early germination stage. 【Method】 Artificial aging treatment with high temperature and high humidity. 【Result】 Artificial aging reduced the germination characteristics and embryo viability of four barley cultivars. The number of small starch granules in seed endosperm increased 72 hours after germination, the number of large starch grains decreased and starch content increased. As the aging time prolonged, the activities of α-amylase, β-amylase and limit dextrinase tended to decrease, and the amylase activity of seed 15 days after aging was significantly lower than that of the control; the content of GA 3 was significantly lower at 24 h after germination In the control, germination 48 ~ 72 h, GA 3 content were higher than the control. ABA content in the germination process are higher than the control. 【Conclusion】 Artificial aging broke the balance between GA 3 and ABA during germination, resulting in the decrease of amylase activity and the slow down of starch decomposition, which in turn led to the decrease of seed vigor and inhibition of germination.