目的:建立用高效液相色谱法快速测定2种大黄游离型蒽醌、结合型蒽醌、番泻苷、鞣质单体4类14种与功效紧密相关组分含量方法,为全面评价大黄药材质量提供依据。方法:采用高效液相色谱法测定,Agilent Zorbax SB-C18色谱柱(4.6mm×250 mm,5μm);流动相Ⅰ:0.1%磷酸水-甲醇梯度洗脱,流动相Ⅱ:四氢呋喃-水-冰醋酸(2∶80∶1.5)与乙腈梯度洗脱,流动相Ⅲ:0.5%冰醋酸水-甲醇梯度洗脱;流速分别为1.0,0.8,1.0 mL.min-1,检测波长分别为254,350,270 nm,柱温25℃。结果:测定了两种大黄的4类功效组分含量,各组分均在30 min内均得到良好分离;不同成分在各自的线性范围内线性关系良好,平均回收率均在97.09%～103.2%,RSD均在0.15%～3.0%(n=6)。结论:方法简便、准确、重复性好,可作为大黄全面质量控制。 OBJECTIVE: To establish a HPLC method for the rapid determination of two kinds of rhubarb free anthraquinone, anthraquinone anthraquinone, sennoside, tannin 14 kinds of 14 kinds and efficacy of closely related components content method for the comprehensive evaluation of rhubarb Quality basis. Methods: High performance liquid chromatography (HPLC) was used on an Agilent Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm); mobile phase Ⅰ: gradient elution with 0.1% phosphoric acid in water and methanol; mobile phase Ⅱ: Acetic acid (2: 80: 1.5) and acetonitrile gradient, mobile phase Ⅲ: gradient elution with 0.5% glacial acetic acid in water and methanol. The flow rates were 1.0, 0.8 and 1.0 mL · min- Column temperature 25 ℃. Results: The four components of rhubarb were determined. The components were well separated within 30 min. The linearity of the two components was good and the average recoveries ranged from 97.09% to 103.2% , RSD 0.15% ~ 3.0% (n = 6). Conclusion: The method is simple, accurate, reproducible and can be used as a total quality control of rhubarb.