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目的 :观察诱导疗程前、疗程中及缓解期 3个时期内B系急淋患儿外周血T淋巴细胞DNA体外合成能力的变化情况。方法 :应用3 H TDR掺入法。结果 :在B系急淋未经治疗的情况下 ,T淋巴细胞的DNA体外合成受到抑制。在诱导疗程后期 ,T淋巴细胞的DNA体外合成能力仍然底下。在缓解期 ,T淋巴细胞的DNA体外合成能力明显增强 ,甚至略高于正常水平。结论 :B系急淋患儿在自然状态下T淋巴细胞转化水平显著低下 ;在诱导疗程后期 ,T淋巴细胞转化水平仍然低下 ;在治疗后缓解期T淋巴细胞转化水平恢复正常 ,甚至出现反跳现象。同时 ,我们认为无限增殖的肿瘤细胞和大剂量的化疗药物是影响患儿T细胞功能的主要因素。
OBJECTIVE: To observe the changes of in vitro DNA synthesis ability of peripheral blood T lymphocytes in children with B-type acute lymphocytic leukemia before induction, during the course of treatment and during the remission period. Methods: 3 H TDR incorporation method was used. RESULTS: DNA synthesis in T lymphocytes was inhibited in untreated B-lymphocytes. In the late induction period, T lymphocytes in vitro DNA synthesis capacity is still below. In remission, T lymphocytes in vitro DNA synthesis capacity was significantly enhanced, or slightly higher than normal levels. CONCLUSION: The T lymphocyte transformation level is significantly lower in children with B-mode acute lymphoblastic leukemia at the natural state. The transformation level of T-lymphocytes is still low in the late stage of induction therapy. The level of T-lymphocyte transformation returns to normal after the treatment, even rebound phenomenon. At the same time, we believe that unlimited proliferation of tumor cells and high doses of chemotherapy drugs are the main factors affecting children’s T cell function.