论文部分内容阅读
以温敏型黄瓜‘C09-123’为试材,通过对不同夜温处理的黄瓜幼叶进行差异蛋白质鉴定,筛选出一个与低夜温影响黄瓜雌性形成密切相关的蛋白,采用生物信息学方法,对该蛋白进行生物信息学分析,并运用基因克隆技术克隆了该基因,以期为明确该基因在黄瓜性别分化中的作用机制奠定基础,也为进一步试验研究提供基因材料。结果表明:该基因全长870bp,编码289个氨基酸,该基因编码的蛋白质属于叶绿体中一个不稳定的疏水蛋白质,无明显信号肽,无跨膜结构。与甜瓜为同一进化分支,同源性较高。该蛋白属于PAPfibrillin蛋白家族,因此将编码该蛋白的基因命名为CsPAP-fib。
A temperature sensitive cucumber’C09-123’was used as material to identify the differential proteins of cucumber young leaves under different night temperature conditions. A protein closely related to the formation of female in low night temperature cucumber was screened out. Bioinformatics methods , The bioinformatics analysis of the protein, and the cloning of the gene by gene cloning technology, in order to clarify the gene in cucumber sexual differentiation lay the foundation for the mechanism, but also for further experimental research to provide genetic material. The results showed that this gene was 870bp in length and encoded a protein of 289 amino acids. The protein encoded by this gene belongs to an unstable hydrophobic protein in chloroplast, with no obvious signal peptide and no transmembrane structure. And melon for the same evolutionary branch, higher homology. This protein belongs to the PAPfibrillin family of proteins, so the gene encoding this protein is named CsPAP-fib.