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目的:探讨灰树花提取物对脾虚小鼠的免疫调节机制。方法:健康雄性清洁级昆明小鼠30只随机分为6组,即空白对照组、脾虚组、阳性对照组(香菇多糖,2 mg·kg-1)、灰树花提取物低剂量组(5 mg·kg-1),灰树花提取物中剂量组(10 mg·kg-1)、灰树花提取物高剂量组(20 mg·kg-1),每组5只,滤菌后ip 10 d,第11天处死动物。检测小鼠腹腔巨噬细胞吞噬中性红、产生一氧化氮(NO)能力(Griess法),以及MTT法检测淋巴细胞转化率、自然杀伤细胞(NK细胞)杀伤活性。结果:高剂量灰树花提取物可明显提高小鼠腹腔巨噬细胞吞噬中性红能力(P<0.05)和产生NO能力(P<0.01);高剂量灰树花提取物能明显提高小鼠脾脏B细胞增殖能力(P<0.01);中、高剂量灰树花提取物能明显提高小鼠脾脏T细胞增殖能力(P<0.05和P<0.01)灰树花提取物中、高剂量组小鼠脾脏NK细胞杀伤能力均明显高于脾虚组(P<0.01)。结论:灰树花提取物可以通过提高巨噬细胞吞噬能力和产生NO能力、以及淋巴细胞转化率、NK细胞杀伤活性的途径提高脾虚小鼠的免疫功能。
Objective: To investigate the immunomodulatory mechanism of Grifola frondosa extract on spleen deficiency mice. Methods: Thirty healthy male Kunming mice were randomly divided into six groups: control group, spleen deficiency group, positive control group (lentinan, 2 mg · kg -1), Grifola frondosa low dose group (5 mg · kg-1), middle-dose group of Grifola frondosa (10 mg · kg-1) and high-dose group of Grifola frondosa (20 mg · kg-1) On day 10, animals were sacrificed. The peritoneal macrophages of mice were swallowed with neutral red for the production of nitric oxide (NO) (Griess method), and the activity of lymphocyte transformation and natural killer (NK) cytotoxic activity were detected by MTT assay. Results: High dose Grifola frondosa extract could significantly increase the ability of phagocytic neutral red (P <0.05) and produce NO in mice peritoneal macrophages (P <0.01) (P <0.01). Middle and high doses of Grifola frondosa extract could significantly increase the T cell proliferation ability of spleen (P <0.05 and P <0.01) The cytotoxicity of murine spleen NK cells were significantly higher than that of spleen deficiency group (P <0.01). Conclusion: Grifola frondosa extract can enhance the immune function of mice with spleen deficiency by increasing phagocytosis of macrophages and production of NO, as well as the pathway of lymphocyte transformation rate and NK cell killing activity.