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在眼科领域的实验研究中,微量蛋白的定量分析是进行生化研究的重要手段之一。经典的“劳里”氏测定法已远远不能满足眼科临床及研究的需要,因它费时,操作繁琐,最大的缺点是采用样品量多,不能测定蛋白含量在10微克以下的样品。本法是利用考马斯亮兰G250与蛋白结合后,颜色的最大吸收波长由463nm变为595nm的特性来测定蛋白质含量的。其优点是: 1.可测定蛋白质含量在10微克以下的样品。2.省时,操作方便,30分钟可完成88个样品的全部操作和测定,比劳里氏法节省一半时间。3.样品用量少,仅用10—50微升,比劳里氏法节省95%样品。4.由于取样少,可用于测定玻璃体、房水及泪液的微量蛋白质。方法:
In the field of ophthalmic research, the quantitative analysis of trace proteins is one of the important means of biochemical research. The classic “Laurie” method has been far from meeting the clinical and research needs of ophthalmology. Because of its time-consuming and cumbersome operation, the biggest drawback is the use of large sample volumes, which can not determine samples with a protein content below 10 micrograms. This method is the use of Coomassie brilliant blue G250 and protein binding, the maximum absorption wavelength of the color from 463nm to 595nm properties to determine the protein content. Its advantages are: 1. Determination of protein content of 10 micrograms below the sample. 2. Time-saving, easy to operate, 30 minutes to complete the 88 samples of all the operations and determination, save half the time than Laurie’s method. 3. Small amount of sample, only 10-50 microliters, save 95% of samples than Laurie’s sample. 4. Due to less sampling, can be used to determine the vitreous, aqueous humor and tear trace protein. method: