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以小麦玉米杂交产生的小麦单倍体为材料,研究了单倍体苗分蘖节切口预处理对加倍率的影响。结果表明,加倍前对分蘖节进行切口处理的单倍体苗加倍率和加倍效率均达90.3%,比对照高16个百分点。对560个加倍成功的DH株的第一结实穗位分布进行了统计,从主茎开始结实的植株只占31.1%,其余均从分蘖穗开始结实,个别植株到第13个分蘖穗才收到种子;前5个穗位开始结实的植株占82.1%,前7个穗位占94.3%。结果表明,小麦单倍体的加倍率不仅受加倍方法的影响,还与加倍处理前后的栽培技术有关,培育多蘖壮苗、提高分蘖成穗率是提高加倍率的重要保障。
The wheat haploid hybridization wheat-maize as material, the haploid seedling tiller section pretreatment on the doubling rate. The results showed that doubling rate and doubling efficiency of haploid seedlings doubling before tillering stage reached 90.3%, which was 16% higher than that of the control. The distributions of the first strong ear of 560 doubled DH strains were statistically analyzed. Only 31.1% of the plants were stubbled from the main stem, and the rest began to grow from tillering panicles to the 13th tiller panicle of individual plants Seeds; The first five ears of the plant began to account for 82.1%, the first seven ears accounted for 94.3%. The results showed that the doubling rate of wheat haploid was not only affected by doubling method but also related to the cultivation techniques before and after doubling treatment. It was an important guarantee to increase the tillering rate by cultivating more tillers and strong seedlings.