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目的 :建立一种快速测定人血浆中氟西汀及其代谢产物去甲氟西汀的反相高效液相色谱法。方法 :用正己烷 -乙腈(98∶2 )提取血浆样品中氟西汀、去甲氟西汀及氯丙帕明 (内标 ) ,在EclipseXDB -C8柱上 ,以 0 0 5mol·L-1磷酸二氢钾 -乙腈 -甲醇 (5 5∶40∶5 )为流动相进行分离 ,流速 1 2mL·min-1,于 2 2 6nm检测。结果 :线性范围为 10～ 5 0 0ng·mL-1,氟西汀和去甲氟西汀相对回收率分别为 99 1%～ 10 4 2 %和 98 2 %～ 10 0 3 % ,日内RSD分别为 4 1%～ 4 8%和 4 5 %～5 8% ,日间RSD分别为 3 5 %～ 5 9%和 4 9%～ 6 8%。结论 :本法简便、精密度高、重现性好 ,结果准确可靠。
Objective: To establish a rapid determination of fluoxetine in human plasma and its metabolite norfluoxetine by RP-HPLC. Methods: Fluoxetine, norfloxacin and chlorpromaplatin (internal standard) were extracted from plasma samples with n-hexane-acetonitrile (98: 2), and eluted with 0 0 5 mol·L-1 Potassium dihydrogen phosphate - acetonitrile - methanol (5 5:40:5) as the mobile phase separation, the flow rate of 12mL · min-1, detected at 226nm. Results: The linear range was 10 ~ 500 ng · mL-1. The relative recoveries of fluoxetine and norfluoxetine were 99 1% -104 2% and 98 2% -103% respectively. The intra-day RSD Were 41% ~ 48% and 45% ~ 58% respectively. The intraday RSD were 35% ~ 59% and 49% ~ 68%, respectively. Conclusion: This method is simple, accurate, reproducible and accurate.