Investigating the effects of residue chemical composition on soil labile organic carbon(LOC) will improve our understanding of soil carbon sequestration.The effects of maize residue chemical composition and soil water content on soil LOC fractions and microbial properties were investigated in a laboratory incubation experiment.Maize shoot and root residues were incorporated into soil at 40%and 70% ?eld capacity.The soils were incubated at 20?C for 150 d and destructive sampling was conducted after 15,75,and 150 d.Respiration,dissolved organic carbon(DOC),hot-water extractable organic carbon(HEOC),and microbial biomass carbon(MBC)were recorded,along with cellulase and β-glucosidase activities and community-level physiological pro?les.The results showed that the cumulative respiration was lower in root-amended soils than in shoot-amended soils,indicating that root amendment may be bene?cial to C retention in soil.No signi?cant differences in the contents of DOC,HEOC and MBC,enzyme activities,and microbial functional diversity were observed between shoot- and root-amended soils.The high soil water content treatment signi?cantly increased the cumulative respiration,DOC and HEOC contents,and enzyme activities compared to the low soil water content treatment.However,the soil water content treatments had little in?uence on the MBC content and microbial functional diversity.There were signi?cantly positive correlations between LOC fractions and soil microbial properties.These results indicated that the chemical composition of maize residues had little in?uence on the DOC,HEOC,and MBC contents,enzyme activities,and microbial functional diversity,while soil water content could signi?cantly in?uence DOC and HEOC contents and enzyme activities. Investigating the effects of residue chemical composition on soil labile organic carbon (LOC) will improve our understanding of soil carbon sequestration. The effects of maize residue chemical composition and soil water content on soil LOC fractions and microbial properties were investigated in a laboratory incubation experiment. Maize shoot and root residues were incorporated into soil at 40% and 70%? Eld capacity. The soils were incubated at 20 ° C for 150 days and destructive sampling was conducted after 15, 75, and 150 days d. Respiration, dissolved organic carbon DOC), and hot-water extractable organic carbon (HEOC), and microbial biomass carbon (MBC) were recorded, along with cellulase and β-glucosidase activities and community-level physiological pro? Les.The results showed that the cumulative respiration was lower in root-amended soils than in shoot-amended soils, indicating that root amendment may be bene? cial to C retention in soil. No signi? cant differences in the contents of DOC, HEOC and MBC, ities, and microbial functional diversity were observed between shoot- and root-amended soils. The high soil water content treatment signi? cantly increased the cumulative respiration, DOC and HEOC contents, and enzyme activity compared to the low soil water content treatment. However, the soil water content diet had little in? uence on the MBC content and microbial functional diversity.There were signi? cantly positive correlations between LOC fractions and soil microbial properties. These results indicated that the chemical composition of maize residues had little in? uence on the DOC, HEOC, and MBC contents, enzyme activities, and microbial functional diversity, while soil water content could signi? cantly in DOC and HEOC contents and enzyme activities.