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我国用A-549和VeroE6细胞从啮齿类动物和病人血清中分离流行性出血热(EHF)病毒,相继获得成功。但上述分离到的毒株均已通过传代细胞不同代次的传代,不宜作为EHF疫苗的筛选毒株。1983年EHF流行季节,我们在流行区采集了急性期(发病2～5天以內)病人血清用大白鼠乳鼠原代肺细胞(RL)同样分离到EHF病毒。典型EHF病人急性期血液按常规方法
In China, A-549 and VeroE6 cells were used to isolate epidemic hemorrhagic fever (EHF) virus from rodents and patient serums, one after another. However, all of the above isolated strains have passed the passage of different generations of passage cells and should not be used as screening strains for EHF vaccine. 1983 EHF epidemic season, we collected in the epidemic area of the acute phase (onset of 2 to 5 days) patient serum with primary rat lung cells (RL) also isolated EHF virus. Acute EHF patients with blood in the conventional method