论文部分内容阅读
目的:通过研究巴西苏木素在大鼠体内的代谢产物,探析其代谢途径。方法:采用UPLC-Q-TOF-MS/MS对灌胃巴西苏木素(30 mg·kg-1)后大鼠的生物样品(粪便、尿液、血浆、胆汁)进行分析,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.4 mL min-1,电喷雾离子源,负离子模式,信息依赖性采集模式进行检测,离子喷雾电压-4.5 k V,离子源温度500℃,碰撞电压100 eV,碰撞能量-35 eV,锥孔气流50 L·h-2,mA 100~1 100,使用PeakView 1.2软件进行数据处理,提取给药样品的总离子流图谱与空白生物样品对比,通过保留吋间、精确相对分子质量及二级质谱的比较与分析,筛选并鉴定其可能的代谢产物。结果:共检测出23个代谢产物,这23个代谢产物是巴西苏木素在大鼠体内甲基化、脱氧、缩氢、羧酸化、水合作用、硫酸化、葡糖糖醛酸化后产生的,其中以硫酸化、葡糖糖醛酸化为主。结论:本实验首次对巴西苏木素在大鼠体内的代谢情况进行研究,能够为该药物的药效学和药理学研究提供一定的物质基础。
OBJECTIVE: To study the metabolic pathway of hematoxylin in rats by studying its metabolites. Methods: The biological samples (stool, urine, plasma and bile) of rats after intragastric administration of hematoxylin (30 mg · kg-1) were analyzed by UPLC-Q-TOF-MS / MS. The mobile phase consisted of 0.1% formic acid - acetonitrile gradient elution, flow rate 0.4 mL min-1, electrospray ion source, negative ion mode, information-dependent acquisition mode, ion spray voltage -4.5 kV, ion source temperature 500 ℃, collision voltage 100 eV, collision energy -35 eV, cone flow 50 L · h-2, mA 100 ~ 1 100, using PeakView 1.2 software for data processing, extraction of the total ion flux profile of drug samples compared with the blank biological samples, by keeping the time, the exact relative Molecular mass and secondary mass spectrometry comparison and analysis, screening and identification of possible metabolites. Results: A total of 23 metabolites were detected. These 23 metabolites were produced after methylated, deoxygenated, hydrogenated, carboxylated, hydrated, sulfated and glucuronidated of hematoxylin in Brazil, Among them, sulfated, glucuronidation dominated. Conclusion: This study is the first to study the metabolism of hematoxylin in rats, which can provide some material basis for pharmacodynamics and pharmacology of this drug.