目的 :对 12 17名运动员尿中 8种类固醇激素的含量进行测定及统计分析 ,以考察我国运动员内源性类固醇激素水平。方法 :对尿样进行预提取、水解、萃取、衍生化等前处理 ,采用GC/MS方法分离检测 ,测定内源性类固醇激素含量。结果 :我国男运动员尿中类固醇水平一般高于女性 ,但都普遍低于国外统计数据 ,尤其是睾酮和 5 β雄烷二醇的水平低于国外数据 4～ 7倍。某些类固醇含量的比值 (如An/Etio、T/ET、5α -diol/5 β -diol)较为恒定 ,且受性别因素影响较小 OBJECTIVE: To determine the content of 8 steroid hormones in 12 17 athletes and to analyze the statistic analysis of endogenous steroid hormones in Chinese athletes. Methods: The urine samples were pretreated, hydrolyzed, extracted, derivatized and other pretreatment. The contents of endogenous steroid hormones were determined by GC / MS. Results: Urinary levels of steroids in Chinese male athletes were generally higher than those in females, but both were generally lower than foreign statistics. The levels of testosterone and 5-β-androstanediol were 4 to 7 times lower than those of foreign countries. Certain steroid content ratios (eg An / Etio, T / ET, 5α -diol / 5 β -diol) are more constant and less affected by gender