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了解在实际生产中常用普通级啮齿类实验动物中清洁级规定排除的 4种主要细菌 (沙门菌、鼠棒状杆菌、嗜肺巴斯德杆菌、支气管败血性鲍特菌 )的携带状况 ,以及验证筛选的实验动物细菌检测常用普通培养基和生化培养基的应用效果。方法 无菌采集动物气管分泌物 ,接种血平皿 ;采集回盲部内容物接种DHL、SS琼脂平皿 ,37℃ 48h后挑取可疑菌落进行纯化 ,取纯培养物进行染色镜检、生化特性鉴定及血清学反应。结论 KM小鼠嗜肺巴斯德杆菌阳性率最高 ,为 6 0 % ;豚鼠携带支气管败血性鲍特菌 ,阳性率为 30 % ;总的细菌检出率为 2 0 % (其中嗜肺巴斯德杆菌总阳性率为 16 2 5 % ,支气管败血性鲍特菌总阳性率为 3 75 % ) ;选出的培养基应用于实验检测中是可行的。
To understand the carrying status of the four major bacteria (Salmonella, Corynebacterium parvum, Pneumocystis pneumophila, and bronchopulmonary bronchiseptica), which are excluded from the clean-level tests commonly used in ordinary rodent experimental animals in practice, and to verify Screening of laboratory animals commonly used common bacteria and biochemical culture medium test results. Methods The tracheal secretions of animals were collected aseptically and inoculated with blood plate. The contents of the ileocecal part were collected and inoculated with DHL and SS agar plates. After 48 hours at 37 ° C, suspicious colonies were picked for purification. The pure cultures were stained for microscopic examination and biochemical characterization. Serological reaction. Conclusions The positive rate of Pneumobacillus pneumophila in KM mice is the highest (60%). In guinea pigs, the positive rate of bronchopulmonary bronchial bacteria is 30%. The total detection rate of bacteria is 20% The total positive rate of Bacillus was 16 2 5%, and the total positive rate of Bordetella bronchiseptica was 3 75%). It was feasible to use the selected medium in the experiment.