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目的:建立固冲止血颗粒的质量标准。方法:采用薄层色谱法(TLC)鉴别炙黄芪、炒升麻、远志、三七、地黄、女贞子;采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定黄芪甲苷含量。结果:TLC色谱中检出炙黄芪、炒升麻、远志、三七、地黄、女贞子的特征斑点;黄芪甲苷在1.1～6.6μg范围内线性关系良好,平均回收率为97.34%,RSD为1.08%(n=6)。结论:本文建立的定性、定量方法简便、准确、可靠,可作为本制剂的质量控制方法。
Objective: To establish the quality standard of Guchong hemostatic granules. Methods: TLC, TLC, Radix Astragali, Radix Polygonatum, Radix Notoginseng, Radix Rehmanniae and Fructus Ligustri Lucidi were determined. The content of Astragaloside Ⅳ was determined by HPLC-ELSD . Results: The spots of Radix Astragali, Radix Scutellariae, Polygala Tengensis, Radix Notoginseng, Radix Rehmanniae and Fructus Ligustri Lucidi were detected in TLC chromatogram. Astragaloside had a good linearity in the range of 1.1-6.6 μg with an average recovery of 97.34% Was 1.08% (n = 6). Conclusion: The qualitative and quantitative methods established in this paper are simple, accurate and reliable and can be used as quality control methods of this preparation.