论文部分内容阅读
取成年健康雄兔的附睾精子加佛氏佐剂免疫日本大耳白雄兔。免疫后第6周开始检出抗精子抗体,继续免疫6周后取睾丸组织作间接免疫酶和间接免疫荧光观察。结果表明,直接加酶或荧光标记的羊抗兔IgG孵育者呈阴性反应。但用稀释的自体血清孵育后,再加酶或荧光标记的羊抗兔IgG则呈阳性反应。提示血清抗精子抗体不能透过血-睾屏障进入曲细精管内,故不能与曲细精管内成熟的精细胞接触。因此,要通过免疫产生血清抗精子抗体,来达到抑制精子生成的目的,至少对于血睾屏障正常者是不适宜的。
Adult male Japanese rabbits were immunized with epididymal spermatozoa plus Freund’s adjuvant. Antisperm antibodies were detected at the 6th week after immunization. After 6 weeks of continuous immunization, testicular tissues were taken for indirect immunoenzyme and indirect immunofluorescence. The results showed that those incubated directly with enzyme or fluorescently labeled goat anti-rabbit IgG showed a negative reaction. However, after incubation with diluted autologous serum, enzyme- or fluorescently labeled goat anti-rabbit IgG was positive. It is suggested that serum anti-sperm antibodies cannot enter into the seminiferous tubules through the blood-testis barrier and therefore cannot contact mature sperm cells in the seminiferous tubules. Therefore, serum anti-sperm antibodies should be generated by immunization to inhibit sperm production, at least for those with normal blood-brain barrier.