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目的:观察中药复方冠心康对大鼠缺血再灌注损伤诱导凋亡心肌细胞结构形态学的影响。方法:40只SD雄性大鼠随机分为4组,假手术组、MIRI模型组、冠心康组、盐酸地尔硫卓组。分组给药处理7天,采用结扎大鼠心脏左冠状动脉前降支30分钟,再灌注2小时复制大鼠MIRI模型,造模成功后,在MIRI结扎线以下区域至心尖取材,通过常规苏木素-伊红(HE)染色,光镜200倍下用图形分析系统定量观察单位视野全部心肌细胞核平均光密度、平均黑度值、心肌细胞核的直径、心肌细胞的面积和周长、最大直径及最小直径的变化。结果:模型组大鼠心肌细胞结构形态学指标明显下降,与假手术组比较差异有统计学意义(P<0.01);冠心康组和盐酸地尔硫卓治疗组心肌细胞结构形态学明显改善,与模型组比较差异均有统计学意义(P<0.05或P<0.01)。结论:冠心康对大鼠MIRI心肌组织具有明显的保护作用,其作用机制与保护心肌细胞结构形态学有明显的关系。
Objective: To observe the effect of Guanxinkang, a traditional Chinese medicine compound, on the morphological changes of cardiomyocytes induced by ischemia-reperfusion injury in rats. Methods: Forty SD male rats were randomly divided into four groups: sham operation group, MIRI model group, Guanxinkang group and diltiazem hydrochloride group. Group administration for 7 days, the left anterior descending coronary artery ligation in rats for 30 minutes, 2 hours after reperfusion rat MIRI model replication, the model was successful, the MIRI ligature in the following region to the apex drawn through the conventional hematoxylin - Eosin (HE) staining, light microscope at 200 times with a graphical analysis system to quantify the visual field per unit area of ?? the average myocardial cell nucleus average optical density, mean blackness, myocardial cell nucleus diameter, area and perimeter, maximum diameter and minimum diameter The change. Results: The morphological indexes of myocardial cells in model group decreased significantly compared with those in sham operation group (P <0.01). The myocardial morphological changes of Guanxinkang group and diltiazem hydrochloride group were significantly improved. Compared with model group The differences between the two groups were statistically significant (P <0.05 or P <0.01). Conclusion: Guanxinkang has obvious protective effect on myocardium of MIRI in rats, and its mechanism of action is obviously related to the protection of cardiomyocyte structure and morphology.