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目的观察~(60)Co-γ辐照灭菌法对蛇胆陈皮散中牛磺胆酸含量的影响。方法采用高效液相色谱法(HPLC)对不同剂量~(60)Co-γ辐照灭菌的蛇胆陈皮中牛磺胆酸含量进行测定,Agilent Zorbax SB-C18色谱柱(4.6×150 mm,5μm);流动相:乙腈-0.1%磷酸溶液(22∶78),流速1 ml/min,检测波长285 nm,选择0、4、6、10 kgy四个不同辐照剂量对蛇胆陈皮散进行灭菌,比较其牛磺胆酸含量的差异。结果不同剂量的~(60)Co-γ辐照灭菌对蛇胆陈皮中牛磺胆酸含量无明显差异。结论蛇胆陈皮散可采用~(60)Co-γ辐照灭菌法进行灭菌,对其牛磺胆酸含量无影响。
Objective To observe the effect of ~ (60) Co-γ radiation sterilization on the content of taurocholic acid in snake gallbladder and tangerine peel. Methods The content of taurocholic acid in the dried snake gall of different doses of 60Co-γ irradiated by HPLC was determined by Agilent HPLC (Agilent Zorbax SB-C18 column 4.6 × 150 mm, 5μm ). The mobile phase was acetonitrile-0.1% phosphoric acid solution (22:78), the flow rate was 1 ml / min, the detection wavelength was 285 nm, and four different doses of 0,4,6,10 kgy , Compare the difference of its taurocholic acid content. Results There was no significant difference in the content of taurocholic acid in snake gall and dried orange peel with 60 Co-γ irradiation at different doses. Conclusion Shexian Tanpi San can be sterilized by ~ (60) Co-γ radiation sterilization, and has no effect on its taurocholic acid content.