利用Basta抗性选择技术快速检测Bt转基因水稻及杂交后代群体单株。试验表明 ,阳性单株在室内和田间有良好的抗螟虫性能 ;在杂交后代中抗除草剂bar基因和目的基因cryIA(b)紧密连锁与协同表达 ;外源基因在F2和BC1群体中遵循孟德尔分离规律 ;应用杂交、回交育种方法将抗虫基因转育到推广品种中是可行的。本研究为进一步选育双价抗虫和抗除草剂水稻新品种奠定了基础 Rapid Detection of Bt Transgenic Rice and Hybrid Offspring Populations with Basta Resistance Selection Technique. The test showed that the positive plants had good resistance to stem borers both indoors and in the field. In the progeny of hybrids, the herbicide resistant bar gene and the cryIA (b) gene were closely linked and co-expressed, and the exogenous genes were followed in F2 and BC1 populations Del’s law of separation; the application of hybridization, backcross breeding methods to insect-resistant genes to promote varieties is feasible. This study laid the foundation for further selection of new varieties of bivalent insect-resistant and herbicide-resistant rice