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目的:检测胰岛素样生长因子结合蛋白7(IGFBP7)在皮肤黑素瘤组织和细胞系中的表达。方法:免疫组化法检测25例原发性皮肤黑素瘤、5例转移性皮肤黑素瘤、20例良性色素痣、10例正常皮肤组织和4株人黑素瘤细胞系中IGFBP7的表达。结果:IGFBP7在正常皮肤和良性色素痣细胞浆中的阳性表达率为85%,而在皮肤黑素瘤胞浆中的阳性表达率为16%和20%。除人黑素瘤细胞系MV3阳性表达IGFBP7外,其余细胞系均阴性表达。结论:IGFBP7在皮肤黑素瘤组织和细胞系中的低表达或功能缺失可能参与了皮肤黑素瘤的发病。
AIM: To detect the expression of insulin-like growth factor binding protein 7 (IGFBP7) in cutaneous melanoma tissues and cell lines. Methods: Immunohistochemistry was used to detect the expression of IGFBP7 in 25 cases of primary cutaneous melanoma, 5 cases of metastatic cutaneous melanoma, 20 cases of benign pigmented nevus, 10 cases of normal skin tissue and 4 human melanoma cell lines . Results: The positive expression rate of IGFBP7 in cytoplasm of normal skin and benign pigmented nevus was 85%, while the positive expression rate in skin cytoplasm of melanoma was 16% and 20%. Except that the human melanoma cell line MV3 positively expressed IGFBP7, all the other cell lines were negatively expressed. Conclusion: The low expression or loss of IGFBP7 in cutaneous melanoma tissues and cell lines may be involved in the pathogenesis of cutaneous melanoma.