论文部分内容阅读
为探讨血管再狭窄发生过程中血浆组织型纤溶酶原激活物及其抑制剂活性与胶原转换及骨桥蛋白基因表达的变化 ,采用发色底物法检测组织型纤溶酶原激活物及其抑制剂活性 ;应用Northern印迹观察骨桥蛋白基因表达活性。结果表明 ,大鼠主动脉内皮剥脱后 3天 ,血浆组织型纤溶酶原激活物活性开始升高 ,第 7天达高峰 ,由对照组的 2 96 .1± 12 0 .2IU L上升为 85 6 .2± 195 .3IU L ,之后随内皮剥脱时间的延长虽有所下降 ,但在所观察的时间范围内 ,均显著高于对照水平 (P <0 .0 5 )。羟脯氨酸测定结果显示 ,内皮剥脱后 3天血管壁胶原降解开始增加 ,第 7天达高峰 ,是对照组的 1.6倍。此外 ,大鼠主动脉内皮剥脱诱导了骨桥蛋白基因表达 ,内皮剥脱后 3天其表达活性为对照组的 5倍。提示在血管再狭窄发生过程中 ,血浆组织型纤溶酶原激活物和骨桥蛋白参与血管重构过程
To investigate the changes of plasma tissue-type plasminogen activator and its inhibitor activity and collagen conversion and osteopontin gene expression during the process of vascular restenosis, the chromogenic substrate method was used to detect the expression of tissue-type plasminogen activator and Its inhibitor activity; Northern blot analysis of osteopontin gene expression activity. The results showed that 3 days after aortic endothelial exfoliation in rat plasma tissue plasminogen activator activity began to rise, reached a peak on the 7th day, from the control group 2 96.1 ± 12.2 IU L increased to 85 6.22 ± 195.3 IU L, and then with the extension of endothelial stripping time although the decline, but in the observed time range, were significantly higher than the control level (P <0.05). Hydroxyproline assay showed that collagen degradation began to increase 3 days after endothelial exfoliation and peaked on the 7th day, which was 1.6 times that of the control group. In addition, rat aortic endothelial exfoliation induced osteopontin gene expression 3 days after endothelial stripping expression activity of the control group 5 times. Tip in the process of vascular restenosis, plasma tissue-type plasminogen activator and osteopontin involved in vascular remodeling process