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背景与目的:尽管能够在70%～90%的恶性肿瘤细胞中可检测到端粒酶活性,但其活性与恶性肿瘤患者预后的关系仍存在争议。本研究旨在探讨端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)在结肠癌组织中表达的临床意义。方法:用定量实时RT-PCR检测59例大肠癌标本的癌组织和癌旁非癌组织hTERT表达。结果:癌组织和癌旁非癌组织hTERT表达与临床病理学特征无相关性。32例DukesA和B期患者中,18例(56%)癌组织hTERT表达低于0.6,14例(44%)表达高于0.6,前者的预后较后者好。20例(62%)癌组织/癌旁非癌组织hTERT表达差异低于0.5,12例(38%)表达差异高于0.5,前者的预后较后者好。27例DukesC和D期患者中,癌组织hTERT表达和癌组织/癌旁非癌组织hTERT表达差异对预测患者的预后无意义。结论:结肠癌组织hTERT表达和癌组织/癌旁非癌组织hTERT表达差异可作为DukesA和B期结肠癌患者预后的指标之一。
BACKGROUND & AIM: Although telomerase activity can be detected in 70% to 90% of malignant tumor cells, its relationship with the prognosis of patients with malignant tumors remains controversial. This study aimed to investigate the clinical significance of the expression of human telomerase reverse transcriptase (hTERT) in colon cancer. Methods: The expression of hTERT in 59 cases of colorectal cancer and adjacent non-cancerous tissues was detected by quantitative real-time RT-PCR. Results: There was no correlation between the expression of hTERT in cancerous tissues and adjacent non-cancerous tissues and the clinicopathological features. Of the 32 DukesA and B patients, the expression of hTERT was lower than 0.6 in 18 cases (56%) and higher than 0.6 in 14 cases (44%), the prognosis of the latter was better than the latter. The difference of expression of hTERT in 20 cases (62%) of cancerous tissues / adjacent non-cancerous tissues was lower than 0.5, while the difference of expression of hTERT in 12 cases (38%) was higher than 0.5. The former had better prognosis than the latter. 27 cases of DukesC and D patients, hTERT expression in cancer tissue and adjacent non-cancerous tissue cancer / hTERT expression differences in the prediction of prognosis of patients is not meaningful. Conclusion: The difference of hTERT expression in colon cancer tissues and hTERT expression in non-cancerous tissues / cancerous tissues can be used as one of the prognostic factors in patients with DukesA and B colon cancer.