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大肠杆菌O157H7是近年来新认识的肠道疾病病原体。随着认识的深入,对大肠杆菌O157H7实验诊断显得尤为重要。本文对近年来大肠杆菌O157H7的细菌培养、免疫磁分离法、ELISA、热解质谱测定、多聚酶链反应等方法的研究情况作了介绍,并对其在流行病学和临床上的应用作了讨论。
Escherichia coli O157H7 is a newly recognized pathogen of intestinal diseases in recent years. With the deepening of understanding, the experimental diagnosis of E. coli O157H7 is particularly important. In this paper, recent researches on bacterial culture, immunomagnetic separation, ELISA, pyrolysis mass spectrometry and polymerase chain reaction of E. coli O157H7 are introduced, and its application in epidemiology and clinical are discussed .