论文部分内容阅读
目的:通过研究原发性肝癌和癌旁组织中NK细胞表面受体活化性及抑制性受体的表达,分析探讨这两种受体含量变化在原发性肝癌发生发展中的关系及其临床价值。方法:通过流式细胞术及免疫组织化学方法检测52例原发性肝癌组织及其癌旁组织中NK细胞数及其活化性、抑制性受体的表达,并结合临床相关因素进行统计学分析。结果:原发性肝癌的NK细胞数量明显低于癌旁对照组(P<0.01),原发性肝癌组织活化性受体NKG2D、NKP44明显低于癌旁组织(P<0.05),而抑制性受体CD158b、CD159a明显高于癌旁组织(P<0.05)。NKG2D、NKP30、NKP44的表达与肝癌临床分期负相关,即在早中期的患者含量较高,越晚期患者肝癌组织中NKG2D、NKP30、NKP44含量越低(P<0.05),NK细胞中抑制性受体CD158b、CD159a的含量在越晚期患者肝癌组织中含量越高(P<0.05)且与AFP高低有关及HBsAg的感染有联系。而NK受体的表达在是否有远处转移、肿瘤分化程度之间以及不同的病灶大小之间差异无统计学意义(P>0.05)。结论:原发性肝癌发病可能与NK细胞减低及NK细胞活化性受体表达降低、抑制性受体表达升高有关。
OBJECTIVE: To study the relationship between the expression of these two receptors and the occurrence and development of primary hepatocellular carcinoma (HCC) by studying the activation of NK cell surface receptor and the expression of inhibitory receptors on the surface of primary hepatocellular carcinoma value. Methods: The number of NK cells and their activation and expression of inhibitory receptors in 52 primary hepatocellular carcinoma tissues and adjacent normal tissues were detected by flow cytometry and immunohistochemistry. Statistical analysis was made based on clinically relevant factors . Results: The number of NK cells in primary hepatocellular carcinoma was significantly lower than that in adjacent non-cancerous tissues (P <0.01). NKG2D and NKP44 were significantly lower in primary hepatocellular carcinoma than in adjacent non-cancerous tissues (P <0.05) Receptors CD158b and CD159a were significantly higher than those in paracancerous tissues (P <0.05). The expression of NKG2D, NKP30 and NKP44 was negatively correlated with the clinical stage of HCC, that is, the content of NKG2D, NKP30 and NKP44 in HCC tissues was higher (P <0.05) in patients with advanced stage, and that in NK cells The levels of CD158b and CD159a were higher in patients with advanced liver cancer (P <0.05) and correlated with the level of AFP and the infection with HBsAg. However, there was no significant difference in the expression of NK receptor between distant metastasis, tumor differentiation degree and different lesion size (P> 0.05). Conclusion: The incidence of primary hepatocellular carcinoma may be related to the decrease of NK cells and the decrease of NK cell activation receptor and the increase of inhibitory receptor.