论文部分内容阅读
目的:建立枳实导滞丸中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量测定方法。方法:采用HPLC法,色谱柱:Waters Symmery C_(18)(250mm×4.6mm,5μm),流动相:甲醇-0.1%磷酸水溶液(85:15),检测波长:254nm,流速:1.0ml·ml~(-1)。结果:芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的线性范围分别为0.011～0.226μg(r=0.9998)、0.005～0.092μg(r=0.9999)、0.010～0.194μg(r=0.999 9)、0.011～0.212μg(r=0.9999)、0.005～0.102μg(r=0.9998);浓度范围内进样量与峰面积线性关系良好。平均回收率分别为97.47%(RSD=1.56%)、99.57%(RSD=0.91%)、100.66%(RSD=1.09%)、101.97%(RSD=1.60%)、101.54%(RSD=1.58%)(n=6)。结论:所建方法简单、快速、准确,可用于枳实导滞丸的质量控制。
Objective: To establish a method for the determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Zhishi Zhidao Wan. Methods: The HPLC method was adopted. The chromatographic column was Waters Symmery C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.1% phosphoric acid solution (85:15), the detection wavelength was 254 nm, the flow rate was 1.0 ml · ml ~ (-1). Results: The linear range of aloe-emodin, rhein, emodin, chrysophanol and physcion were 0.011 ~ 0.226μg (r = 0.9998), 0.005 ~ 0.092μg (r = 0.9999), 0.010 ~ 0.194μg = 0.999 9), 0.011-0.212μg (r = 0.9999), and 0.005-0.102μg (r = 0.9998). The linearity of the injection volume and peak area was good in the range of concentration. The average recoveries were 97.47% (RSD = 1.56%), 99.57% (RSD = 0.91%), 100.66% with RSD = 1.09%, 101.97% with RSD = 1.60% and 101.54% with RSD = 1.58% n = 6). Conclusion: The proposed method is simple, rapid and accurate and can be used for the quality control of Zhishi Zhizhi Wan.