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黄萎病是我国向日葵上一种严重的土传维管束病害。本研究针对其特点,对向日葵黄萎病病菌大丽轮枝孢菌的几种常用接种方法进行了测试。结果表明,其病原菌8种接种方法以接种孢子量为1×107cfu/m L时所产生的发病程度最重。采用蘸根接种法发病率最高,可达到82.7%,病情指数62.1,发病所需时间为14.33 d;叶片针刺涂抹方法发病最快,时间最短为13.67 d,发病率为79.8%,病情指数60.5,且发病均匀。两种方法具有重复性好、试验周期短、鉴定效率高等优点,且操作简便、材料易得。
Verticillium wilt is a serious soil-borne vascular bundle disease on sunflower in China. In this study, several commonly used methods of inoculation of Verticillium dahliae on Verticillium dahliae were studied according to their characteristics. The results showed that the eight pathogenic bacteria inoculation method inoculation spores amount of 1 × 107cfu / m L produced the most serious degree of disease. The diuretic root inoculation method had the highest incidence rate of 82.7%, disease index of 62.1 and disease onset time of 14.33 days. The incidence of leaf acupuncture smear method was the fastest, the shortest time was 13.67 days, the incidence rate was 79.8%, the disease index was 60.5 , And the incidence of uniform. The two methods have the advantages of good repeatability, short test period and high identification efficiency, and are easy to operate and obtain materials easily.